PC Linker (photocleavable)
Photocleavable Linker (PC Linker) often used in the design of multi-functional single-stranded nucleotide conjugates for the selection of novel DNA or RNA-based catalysts in vitro. Photocleavable PC linker modified oligonucleotide are demonstrated in Bruker Daltonik's genoSNIP, a MALDI-TOF MS based assay system for SNP detection.
Photocleavable PC linker contained a non-nucleosidic group that can be used to link two nucleotide sequences through a short, UV photo-cleavable C3 spacer arm. Upon irradiation by UV light, photo-cleavage released oligonucleotide contain a 5-phosphorylated group with greater hybridization affinity for a complementary DNA strand than PC spacer.
Photocleavable PC linker can be incorporate at any position within an oligonucleotide. Contact Bio-Synthesis for service details.
XX-15A uv lamp 365nm 臺式紫外線燈
Cleavage occurs by irradiation with near-UV light (300-350 nm, complete cleavage occurs within 5 minutes. Try using a XX-15A UV lamp at a distance of 15 cm (emission peak 365 nm, 300 nm cut-off, 1.1 mW intensity at~31 cm).
研究發現crRNA-c對核酸內切酶降解具有極端穩定性，卻會在輻照度為20 mW/cm2的365nm UV光照射下，在30秒內釋放出線性crRNA，這為響應性CRISPR編輯提供了一個極好的響應窗口。通過更進一步的研究發現，這種技術應用于CRISPR-Cpf1系統也是可行的，這意味著他們的光激活環狀gRNA策略是調控RNA-引導的CRISPR編輯的通用方法。